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製備生物炭輔助念珠菌吸附銅離子之研究 = Study on Preparation of Biochar to Assist Candida tropical to Adsorb Copper Ion / 張力中.

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隨著經濟發展，工廠中產品使用的重金屬元素也相當廣泛，廢水中也會摻雜各種有害重金屬，其中銅大量使用且用途廣泛，大量含銅廢水進入廢水生物處理單元時，會遇到一個嚴重的問題:微生物在水中銅濃度過高的環境中，會遭到抑制，我們可能需要另類的方式去處理含銅廢水，經過文獻研究生物炭(Biochar)的多孔隙及高比表面積能使我們去吸附水中的銅離子，台灣主食為米，每年產生的農業廢棄物高達462噸，大量的稻殼農民多半都使用焚燒處理，或許我們可以有更好的利用方式將稻殼燒製成生物炭去輔助微生物去處理含銅廢水。本實驗利用念珠菌(Candida tropical)和生物炭混合後加入500ppm的硫酸銅(CuSO4)再一起置於震盪培養箱後，拿出培養箱後我們將培養液離心後並過濾，並接連監測3天實驗數據，結果表明念珠菌與稻殼灰皆可吸附水中的銅離子，利用單獨稻殼灰進行吸附時，可以吸附水中部份的銅離子，並且於48小時候達到平衡，再利用單獨念珠菌進行吸附可以將水中銅離子大部分吸附，但會因為念珠菌死亡導致銅離子脫附間接影響吸附效率，最後利用念珠菌混合稻殼灰的方式進行吸附，在前36小時吸附效果最好，吸附後水中銅離子剩餘0.17mg/L，但因為稻殼灰長時間浸泡在培養液內，稻殼灰逐漸裂解導致銅離子脫離吸附，也導致培養液中的銅離子濃度上升，第三天因為新的稻殼灰沉降至培養液內將銅離子吸附，銅離子濃度也逐漸降低。. With the development of the economic, the heavy metal elements used in the products of the factory are also quite extensive. Therefore, the wastewater also be doped with various harmful heavy metals. Among those, copper is used in large quantities and has a wide range of applications. When a large amount of copper-containing wastewater enters the wastewater biological treatment unit, it will encounter a serious problem. The problem such like microorganisms will be inhibited in the environment where the copper concentration in the water is too high. We may need an alternative way to treat the copper-containing wastewater. After literature review, the porosity and high specific surface area of biochar can make to absorbs copper ions in water. The staple food in Taiwan is rice. The annual agricultural waste generated is as high as 462 tons. Most of the rice husk farmers use incineration to treat it. Auxiliary microorganisms to treat copper-containing wastewater. In this experiment, Candida tropical and biochar were mixed with 500ppm copper sulfate (CuSO4) and then placed in a shaking incubator. After taking out the incubator, we centrifuged the culture solution and filtered it for monitored it 3 days. The experimental data shows that both Candida and rice husk ash can absorb copper ions in the wastewater. When using rice husk ash alone for adsorption, it can absorb part of the copper ions in water, and reaches equilibrium within 48 hours. Adsorption can absorb most of the copper ions in the water, but the desorption of copper ions will indirectly affect the adsorption efficiency due to the death of Candida. Finally, the method of mixing Candida with rice husk ash were used for adsorption. The adsorption effect was the best in the first 36 hours. The remaining copper ion in the water is 0.17mg/L. Because of the rice husk ash which soaked in the culture solution for a long time, the rice husk ash gradually cracks. It leads to the desorption of copper ions, which als

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