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胺化石墨烯/石墨烯原子級層狀複合材料應用於溶液式閘極場效電晶體並檢測阿茲海默症生物標誌物p-tau217 = Solution gate field-effect transistor based on aminated graphene /graphene composite for Alzheimer's disease biomarker p-tau217 detection / 林郁修.

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摘要註

阿茲海默症為一種神經退化性疾病，屬於失智症的一種，佔所有患者中60~70%。許多的研究表明，過度磷酸化tau蛋白(p-tau)與乙型澱粉樣蛋白(Aβ 40/42)，兩種蛋白與阿茲海默症有密切的關聯，可將其視為阿茲海默症的重要標誌物，若能在患者早期檢測出低濃度的p-tau與Aβ 40/42，則可以更早投藥以達成症狀的減緩。 本研究使用胺化石墨烯/石墨烯原子級層狀結構之複合材料作為SGGT中的通道材料來檢測p-tau217。胺化石墨烯上層具有胺基、吡咯N官能基結構，可與抗體尾端的羧基產生脫水反應產生共價鍵結，並以直立的方式與胺化石墨烯表面接合，使抗體具有較多位點可捕捉抗原。此實驗利用低壓化學氣相沉積法製備出二維材料雙層石墨烯，並用濕式轉印法轉印至鍍有電晶體電極的二氧化矽基板上方。上層石墨烯經過低損傷電漿改質後形成胺化石墨烯，作為生物探針連接層並偵測標誌物p-tau217。而下層石墨烯則保有原先優異的石墨烯電性傳輸表現。 石墨烯測量電流-閘極電壓時，電流曲線最低點為狄拉克點(Dirac point)，透過量測不同階段的SGGT元件，觀察狄拉克點的位移。結果表明，胺化石墨烯與抗體產生鍵解後狄拉克點發生位移。p-tau217標誌物檢測濃度範圍為: 1 fg/mL-100pg/mL，隨著p-tau217的濃度增加，狄拉克點逐漸向右位移，證明了攜帶正電的抗原造成石墨烯P型摻雜。此感測元件線性度為0.9955，檢測最低濃度可至1 fg/mL。. Alzheimer's disease is a type of neurodegenerative disease and a form of dementia, accounting for 60-70% of all patients. Many studies show that hyperphosphorylated tau (p-tau) and amyloid-β (Aβ 40/42) are closely to Alzheimer's disease. Early detection of low concentrations of p-tau and Aβ 40/42 in patients is crucial. Solution-gated graphene transistor (SGGT) have been proven that has highly sensitive and can measure in liquid environments. In this study, we use aminated graphene /graphene atomic layer composite material as the channel material in a SGGT to detect p-tau217. The aminated graphene has amine and pyrrolic N functional groups, which can form covalent bond with the carboxyl group at the bottom of antibody, it can increase the antibody's position to catch p-tau217. we used chemical vapor deposition to prepare the bilayer graphene. the top layer was modified by low-damage plasma to form aminated graphene as the bio-linker layer to detect the p-tau217 and the bottom layer remain its excellent electrical properties. The results showed that after the antibody bonding to the aminated graphene, the dirac point shifted to the right. the detection range for the p-tau217 was 1 fg/mL to 100 pg/mL. As the concentration of the p-tau217 increased, the dirac point shift to the right, demonstrating that the positively charged antigen caused p-type doping in the graphene. the linearity of the sensing element is 0.9932 and concentrations as low as 1 fg/mL can be measured..

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